Insights into the CRISPR/Cas system of Gardnerella vaginalis

Biotypes of Gardnerella vaginalis.

A simple and reproducible scheme for identifying biotypes of Gardnerella vaginalis has been developed, based on reactions for lipase, hippurate hydrolysis, and beta-galactosidase. Among a total of 359 strains tested, eight biotypes were observed, the most common ones being types 1 (beta-galactosidase positive, lipase positive, hippurate positive), 2 (beta-galactosidase negative, lipase positive...

Identification of Gardnerella vaginalis with the API 20 Strep system.

A total of 137 strains of Gardnerella vaginalis were examined by the API 20 Strep system. The system was shown to be reliable when the tests were compared with standard identification methods, and very little confusion occurred with streptococcal profiles; consequently, G. vaginalis has been included in the API 20 Strep data base.

[Sexual transmission of Gardnerella vaginalis].

Male carriage of Gardnerella vaginalis.

The prevalence of Gardnerella vaginalis in the urethra of 430 men attending a clinic for sexually transmitted disease was 11·4%; it was significantly higher in heterosexuals (14·5%) than in homosexuals (4·5%). There was no evidence of rectal or subpreputial carriage of G vaginalis, and urethral carriage was not associated with symptoms of urethritis.

Identification of Gardnerella (Haemophilus) vaginalis.

Different tests for the identification of Gardnerella (Haemophilus) vaginalis and for its differentiation from catalase-negative unclassified coryneforms from the vagina were evaluated on over 200 bacterial strains, with special emphasis on optimal test conditions. A presumptive identification of G. vaginalis in the clinical laboratory can be made on the basis of colonial morphology, clear beta...